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. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: J Mol Cell Cardiol. 2009 Aug 15;47(4):552–560. doi: 10.1016/j.yjmcc.2009.07.018

Generation of Na,K-β₁ conditional knockout mice. (A) A schematic representation of the targeting strategy. The wild-type ‘Na,K-β₁ locus’ showing exons 1–4 (E1–E4) and the NdeI restriction sites (N) within the sequence is depicted. Exon 2 targeted for deletion is shown in yellow. The ‘Targeting Construct’ shows the selection marker cassette in orange flanked by the loxP sites () and the gene sequence used for homologous recombination (in blue, between the two dotted lines). The sizes of the DNA fragments expected from NdeI digestion and Southern hybridization using the probe () are indicated on the green lines. (B) Southern blot analysis of NdeI digested genomic DNAs isolated from positive ES clones before (Targeted) or after (Floxed) transient transfection with CMV-Cre. (C) PCR analysis of genomic DNAs from positive ES clones. The floxed allele yields a 900 bp PCR product using the indicated primers (), whereas the targeted allele fails to generate any product under the PCR conditions used.

Inline graphic — Generation of Na,K-β₁ conditional knockout mice. (A) A schematic representation of the targeting strategy. The wild-type ‘Na,K-β₁ locus’ showing exons 1–4 (E1–E4) and the NdeI restriction sites (N) within the sequence is depicted. Exon 2 targeted for deletion is shown in yellow. The ‘Targeting Construct’ shows the selection marker cassette in orange flanked by the loxP sites () and the gene sequence used for homologous recombination (in blue, between the two dotted lines). The sizes of the DNA fragments expected from NdeI digestion and Southern hybridization using the probe () are indicated on the green lines. (B) Southern blot analysis of NdeI digested genomic DNAs isolated from positive ES clones before (Targeted) or after (Floxed) transient transfection with CMV-Cre. (C) PCR analysis of genomic DNAs from positive ES clones. The floxed allele yields a 900 bp PCR product using the indicated primers (), whereas the targeted allele fails to generate any product under the PCR conditions used.