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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Biochimie. 2009 Jul 14;91(10):1307–1310. doi: 10.1016/j.biochi.2009.07.001

Figure 2.

Figure 2

Hypoxia (Hy) decreased c-Myc protein levels in both non-tumorigenic Beas-2B and tumorigenic A549 cell lines in a time-dependent manner. Beas-2B and A549 cells were cultured under normoxic (N) or hypoxic (Hy: 1% O2) conditions for 6, 12 and 24 hr. After treatment, cells were lysed and 50 μg of total protein was separated on 7.5% SDS-PAGE gels, followed by Western blotting using antibodies against c-Myc or α-tubulin (to control for loading). Similar data were obtained in at least two other independent experiments; a representative Western blot is shown.