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Cholera toxin B subunit (CTB)–specific (gray squares) and keyhole limpet hemocyanin (KLH)–specific (black squares) IgG memory B cell kinetics. On all days, data points were excluded if peripheral blood mononuclear cells (PBMCs) were poorly stimulated (<4500 total IgG memory B cells/5 × 105 PBMCs cultured). Additionally, data points were excluded if a CTB-specific antibody-secreting cell response was already present before culture and stimulation.
