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. 2009 Sep 30;4(9):e7285. doi: 10.1371/journal.pone.0007285

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Downing et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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MinD oscillation period in 10 mM HEPES buffer at pH 7.0 as function of cumulative exposure time to excitation illumination. The cumulative exposure time represents the sum of the exposure times used for all images taken of a group of bacteria. The time interval between exposures in a sequence of images was 4.5 seconds (to determine the period) and the interval between repeated sequences was 10 minutes (to recover the steady-state response to previous illumination). Repeated exposure of a group of bacteria to the fluorescence excitation light lengthened their average GFP-MinD oscillation period.