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. 2009 Sep 30;4(9):e7285. doi: 10.1371/journal.pone.0007285

Figure 5. Reversibility of period lengthening for divalent cat ions.

Figure 5

(A) Bright field image of E. coli bacteria PB103 in 100 mM Ca++ ions and 10 mM HEPES buffer. The bacteria assume a more rounded shape and exhibit a bright center region. (B) The same bacteria after the suspending medium is replaced with the 10 mM HEPES control. (C) In 100 mM Ca++ the bacterium marked by the arrow in (A) shows a fluorescence that is uniform over its length. Its polar fluorescence is non-oscillatory but decreases slowly with time due to photobleaching (▴). When re-immersed in the control solution, MinD oscillations return for the same bacterium (•, with the solid line as a fit). Open circles indicate fluorescence background levels beside the bacterium (○).