Table 1.

Summary of results and comparison to Biacore results. Kinetichip on rates and dissociation constants were calculated spot by spot and then averaged. Biacore kinetic constants were calculated by a global fit to a concentration series of association and dissociation curves, with dissociation constants determined simply by dividing off rate by on rate. This method did not generate standard deviations, but analysis of the curve-by-curve variation when fitting to the curves individually suggests standard deviations on the order of 10⁻⁴ s⁻¹, 10⁴ M⁻¹ s⁻¹, and 5 nM for the His results, and 2×10⁻³ s⁻¹, 5×10⁴ M⁻¹ s⁻¹, and 30 nM for the T7 results.

	koff±SD (s−1)	kon±SD (M−1 s−1)	Kd±SD (nM)	n
His Kinetichip	3.3×10−4±2.8×10−5	4.4×104±6.5×103	7.5±0.73	240
His Biacore	3.0×10−4	9.8×104	3.1
T7 Kinetichip	1.9×10−3±6.6×10−4	4.4×104±1.0×104	45±14	492
T7 Biacore	8.1×10−3	1.1×105	72
T7 Kinetichip (HBS-EP buffer)	5.7×10−3±8.5×10−4	4.3×104±8.6×103	130±33	120