Figure 5. Deletion of TYE7 results in higher trehalose and glycogen levels.

(A) The trehalose content of wild type (BWP17), tye7, and gal4 cells at both stationary and logarithmic phases were measured. Trehalose levels (nmol trehalose per mg cell protein) were reported relative to the wild type in stationary phase. Both wild type and gal4 cells had no detectable trehalose levels in the logarithmic phase and were not included in the graph. (B) Wild type (BWP17), tye7, and gal4 cells were exposed to iodine vapor to indicate the glycogen content as iodine vapor stains cells brown upon reacting with glycogen. The tye7 cells stained a darker brown than the wild type indicating that there is more glycogen present.