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. 2009 Oct 9;5(10):e1000612. doi: 10.1371/journal.ppat.1000612

Figure 7. Gal4p displays carbon source-dependent binding while Tye7p binds its targets constitutively.

Figure 7

(A) Binding of Gal4p and Tye7p to HXK2 (i), PGK1 (ii), PDA1 (iii), and TPS3 (iv) under glucose, galactose, and glycerol growth conditions with the tiling array. HXK2 and PGK1 represent common glycolytic targets while PDA1 and TPS3 represent Gal4p and Tye7p specific targets, respectively. The ORF and 1500 bp upstream region is shown. Values for each data point were determined by taking the mean fold enrichment of each probe and the surrounding four probes. The dashed blue and green lines represent the Tye7p and Gal4p binding sites, respectively (approximated over the three carbon sources). The consensus motifs and their positions are indicated for each factor. (B) Consensus motifs based on the top 30 peak intensities from the tiling array for Gal4p and Tye7p on glucose media. The sequence surrounding the peak point (covering 5 probes, 300 bp) for each target was sent to MEME (http://meme.sdsc.edu/meme4/) and the most significant motif (Gal4p: E = 9.3E-24; Tye7p: E = 1.8E-15) is reported.