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. 2009 Oct 9;5(10):e1000612. doi: 10.1371/journal.ppat.1000612

Figure 8. TYE7 and GAL4 are important for hypoxic growth and the induction of glycolytic genes in a low oxygen environment.

Figure 8

(A) Cells were serially diluted, plated on YPD, and incubated in an anaerobic chamber at 30°C for 4 days. The chamber was flushed daily with nitrogen. Due to the space limitation of the chamber, the revertant strains were unable to be spotted on the same plate. A second experiment including the revertant strains was done on two separate plates to verify the results (Figure S5B). (B) Heat map displays were created as described in Figure 4. Transcription profiles of selected metabolic genes for (from left to right) BWP17 hypoxia relative to BWP17 normoxia, tye7 hypoxia relative to BWP17 hypoxia, and tye7 normoxia relative to BWP17 normoxia. The expression levels of these and other metabolic genes are given in Table S7. (C) The expression levels of several glycolytic genes were measured by qPCR in the tye7 and gal4tye7 strains relative to the wild type (BWP17) after 30 min of growth under hypoxic conditions. ACT1 was used as the reference.