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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Proteome Res. 2009 Aug;8(8):3944–3950. doi: 10.1021/pr900251d

Figure 1.

Figure 1

Sample preparation for LC/MS/MS runs.

(A) Flow chart for quantifying protein and peptide used for optimization.

(B) Quantification of yeast lysate on a short 9% SDS gel with BSA as standard. The standard curve used for quantification was shown (n = 3).

(C) Peptide quantification by SILAC methodology. Same amount of peptides digested from heavy labeled yeast cells was spiked in the input and the elution fraction, and then analyzed by LC/MS/MS.

(D) Calculation of averaged peptide recovery from 116 peptides that are plotted according to retention time in LC/MS/MS. The value is shown as mean and standard deviation.