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Evaluation of run-to-run variation of LC/MS/MS system.
(A) Base-peak chromatogram of four repeat runs. Peptides derived from 1 μg of yeast lysate were loaded by an autosampler during 15 min (not shown), and then eluted in a 5–35% gradient of buffer B over 45 min (15–60 min).
(B) Comparison of accepted spectrum count (SC), peptides and proteins identified in four repeat runs.
