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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Mol Microbiol. 2005 Oct;58(2):565–579. doi: 10.1111/j.1365-2958.2005.04852.x

Fig. 1. An RSF1010 derivative forms close contacts with six VirB/D4 subunits during translocation. Strain LBA4404 (ΔT-DNA) with or without pML122 was assayed for formation of pML122 contacts with VirB/D4 channel subunits by TrIP.

Fig. 1

A. Control experiment showing PCR amplification of pTiAch5 repAB (Ct) and pML122 mobA (IncQ) fragments in the soluble fraction (S) but not material immunoprecipitated (IP) with anti-VirD2 antibodies from LBA4404 extracts.

B and C. Antibodies to the VirD4 receptor (panel B) and the VirB/VirJ subunits listed on the left (panel C) were used to immunoprecipitate material from extracts of FA-treated LBA4404 and LBA4404(pML122) cells. Both the repAB control (Ct) and pML122 mobA (IncQ) fragments were detected in solubilized material (S) by PCR amplification. The mobA fragment was detected only in the material precipitated with antibodies to VirD4, VirB2, VirB6, VirB8, VirB9 and VirB11.