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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Mol Microbiol. 2005 Oct;58(2):565–579. doi: 10.1111/j.1365-2958.2005.04852.x

Fig. 4. The pSa Osa protein blocks VirD4 receptor access to the T-DNA.

Fig. 4

A. Kalanchoe daigremontiana leaves were scored for tumour formation on a scale of no tumours (−) to WT tumours (+++) 5 weeks after inoculation with WT A348, or A348 derivatives producing Osa from plasmid pUCD3960 (Osa) or VirE2-GFP (E2-GFP) from plasmid pXZ66, or overproducing VirB9, VirB10 and VirB11 from pED9.

B. Effects of Osa and VirE2-GFP production on T-DNA processing, as monitored by precipitation of the T-DNA substrate with antibodies to VirD2 from extracts of the strains listed in A. For the QTrIP data presented in the upper histogram, the amounts of T-DNA recovered from the various strains are presented as a fraction of T-DNA recovered from WT A348 (normalized to 1.0).

C. Effects of Osa and VirE2-GFP production on T-DNA transfer. Lower panels: TrIP data showing amplification of ophDC (Ct) and gene 7 (T-DNA) fragments from soluble fractions (S) and immunoprecipitates (IP) with antibodies to VirD4 (left) or VirB9 (right). Upper panels: QTrIP data showing levels of T-DNA recovered from the various strains.