A. Conjugative transfer frequencies of LBA4404 (ΔT-DNA) donor strains carrying IncQ plasmid pML122ΔKan without or with plasmids producing Osa protein (pUCD3960) or VirE2-GFP (pXZ66). Donors were mated with either WT A348 carrying plasmid pBBR1MCS (Strr). PC1000 (ΔvirB operon) carrying pML122ΔKan served as a donor in a control mating. Data are presented for a representative experiment.
B. Effects of the IncQ plasmid and derivatives on T-DNA transfer. Lower panel: TrIP data showing amplification of pTiAch5 repAB (Ct) and pML122 mobA (IncQ). Upper panel: QTrIP data showing levels of pML122 in the immunoprecipitates recovered with antibodies to VirD4 (left panels) and VirB9 (right panels).