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. Author manuscript; available in PMC: 2010 Aug 14.
Published in final edited form as: Mol Cell. 2009 Aug 14;35(3):352–364. doi: 10.1016/j.molcel.2009.06.015

Figure 4. USP22 deUB activity regulates the ubiquitination and steady state levels of TRF1.

Figure 4

(A) Efficient depletion of the indicated proteins in 293T cells. (B) Increased ubiquitination of TRF1 upon depletion of GCN5, USP22 or ATXN7L3. Cells were transfected with 6xHis-ubiquitin and FLAG-TRF1 expression vectors and the ubiquitinated species were precipitated by Ni-NTA agarose from nuclear extracts. (C) Schematic representation of Cysteine 185 residue conservation among the USP22 orthologs in different species. (D) Expression of wt -USP22 and C185S–USP22 in HeLa cells. (E) Expression of wt-USP22 restores TRF1 protein levels in USP22 depleted cells, while over expression of C185S–USP22 decreases TRF1 protein levels.