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. Author manuscript; available in PMC: 2010 Aug 14.
Published in final edited form as: Mol Cell. 2009 Aug 14;35(3):352–364. doi: 10.1016/j.molcel.2009.06.015

Figure 5. Depletion of hSAGA deUB module induces TIFs and telomere elongation.

Figure 5

(A) GCN5, USP22 or ATXN7L3 depleted cells, as well as control shRNA treated cells, were subjected to immunostaining with anti-53BP1 antibody (green) followed by PNA-FISH (red). 100 cells of each sample were counted, and cells with ≥4 TIFs per nucleus (yellow signals indicated with arrowheads) were scored.

(B) Quantification of the data represented in (A). Error bars represent standard deviation of the mean (n=2) (C) Telomere elongation after depletion of GCN5, USP22 and ATXN7L3 in HeLA cells. Cells stably expressing denoted shRNAs were harvested at the indicated passages and used for telomere restriction fragment Southern analysis. In-gel hybridizations were done using 32P labeled (GGGTTA)4 or (CCCTAA)4 probes. The hybridized gels were exposed to phosphoimager screens.