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. Author manuscript; available in PMC: 2010 Aug 14.
Published in final edited form as: Mol Cell. 2009 Aug 14;35(3):352–364. doi: 10.1016/j.molcel.2009.06.015

Figure 6. Depletion of GCN5 impacts hSAGA integrity.

Figure 6

(A) Depletion of GCN5 in HeLa S3 cells by shRNA leads to lowered TRF1 protein levels.

(B) Depletion of GCN5 compromises association of USP22 and ATXN7L3 with SAGA. SAGA was purified from nuclear extracts prepared from FLAG-HA-SPT3 expressing HeLa S3 cells. Immunoprecipitated fractions as well as nuclear extracts were resolved by SDS-PAGE and blotted with the indicated antibodies. (C) Quantification of the data from multiple experiments like that shown in (B). X-ray films were scanned and the images were quantified using ImageQuant software. Error bars represent standard deviations of the mean (n=4)