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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Circ Res. 2008 Jun 5;103(1):53–60. doi: 10.1161/CIRCRESAHA.108.176321

Figure 4.

Figure 4

An intact GA was important for PKGIγ nuclear localization. (A) Nz and BFA caused GA disassociation and decreased PKGIγ nuclear localization in 8-Br-cGMP-treated RFL-6 cells. (B) cGMP-induced nuclear PKGIγ localization was abolished by Nz exposure and inhibited by BFA treatment. Nuclear colocalization of PKGIβ-FLAG and GFPNLS, shown in the inset, was objectively quantified in digitonin-treated RFL-6 cells as detailed in the text. Results are expressed as means±SD, n=6 per group, and typical of three independent experiments. * and †P<0.05, indicated treatment vs. the other treatment groups.