Fig. 2.

(A) Germination response at 20 °C for immature caryopses (30 DAP) from two sorghum inbred lines: sprouting resistant ‘IS9530’ and sprouting-susceptible ‘RedlandB2’. (B) ABA content in the embryo during incubation of whole caryopses. ABA content was determined by radioimmunoassay. (C) Expression (determined by RT-QPCR; relative to SbActin) of sorghum genes encoding homologues for ABA signalling proteins: transcription factors SbABI4, SbABI5, SbVP1, protein-kinase SbPKABA1 and a PP2C-type protein kinase, SbABI1. SbRAB28 was included as an ABA-responsive gene (see Materials and Methods). Data in (A), (B) and (C) belong to the same experiment (year 2006). Each value is the mean of two biological replicates coming from two independent field plots.