Fig. 2.
Undifferentiated ORMES6 ESCs express embryonic cell markers. (A) SSEA 4 (green); (B) Oct4 (red); (C) glycoprotein TRA-1-60 (green)/ Oct4 (red) and (D) glycoprotein TRA-1-81 (green)/Oct4 (red). The nuclei were stained with DAPI (blue). The undifferentiated ORMES6 ESCs expressed SSEA 4, Oct4, TRA-1-60 and TRA-1-81 antigens. Cells only stained with DAPI were MEF feeder cells. Scale bar: 40 μm.