Skip to main content
. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Differentiation. 2008 Dec 2;77(3):229–238. doi: 10.1016/j.diff.2008.10.014

Fig. 3.

Fig. 3

RT-PCR analysis of gene expression at select stages of differentiation. Oct4, Sox2 and NANOG are markers of pluripotent stem cells. MAP2, β-tublinIII, NeuroD are neuronal markers, and GFAP is glial cell marker. Undifferentiated ORMES6 ESCs expressed Oct4, NANOG, Sox2 stemness genes and MAP2, β-tublinIII neuronal genes. Oct4 and NANOG transcription factors were markedly down-regulated in the rosette cells. Sox2, MAP2, β-tublinIII neuronal genes and GFAP glial cell genes were expressed in the rosette cells. The neural subtype cells derived from rosette cells expressed Sox2, MAP2, β-tublinIII, GFAP and NeuroD genes. However, spontaneously differentiated cells only expressed β-tublinIII, Sox2 and MAP2 genes.

Lane 1: undifferentiated ORMES6 ESCs; Lane 2: neural progenitor cells (neural tube like-rosette cells) induced by neural induction medium (Step 2 of protocol); Lane 3: Neural subtype cells derived from rosette cells (Step 4); Lane 4: Spontaneously differentiated ORMES6 ESCs cultured in ESC medium without MEF cells.