Skip to main content
. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Differentiation. 2008 Dec 2;77(3):229–238. doi: 10.1016/j.diff.2008.10.014

Fig. 5.

Fig. 5

Expression of neuroepithelial markers by neural tube-like rosette cells. After exposure to bFGF for 14–16 days, neural tube-like rosette cells formed in the EBs outgrowth. The isolated rosette cells were positively stained for nestin, FoxD3, Pax6 and Sox10 that are considered as markers for neuroepithelial stem cells. The neural rosette cells were also positive for Ki67, which is a cell proliferation marker. (A) Nestin (green label); (B) FoxD3 (red label); (C) Pax6 (green label); (D) Sox10 (green label); (E) Ki67 (red label) and (F) percentage of each marker. The nuclei were counter-stained with DAPI (blue). Scale bar: 40 μm.