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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Differentiation. 2008 Dec 2;77(3):229–238. doi: 10.1016/j.diff.2008.10.014

Fig. 6.

Fig. 6

Neural subtype cells derived from the rosette cells. The neural subtype cells differentiation was initiated by removal of bFGF from the culture medium. Most differentiated cells expressed neuronal cell markers, a small subpopulation of the differentiated cells expressed glial lineage cells markers. (A) Neuronal marker β-tublinIII (red label); (B) neuronal-specific protein MAP2 (red label); (C) glial cell marker GFAP (red label); (D) glial cell marker S100 (red label); (E) glial cell marker A2B5 (green label) and (F) oligodendrocyte marker O4 (green label). The nuclei are counter-stained with DAPI (blue). Scale bar: 40 μm. (G) The percentages of markers expressed by the differentiated neural subtype cells.