Table 2.

Summary of theoretical adsorption footprint values and loading of a mAb monolayer adsorbed to a surface for different model scenarios. The models are organized from small to large footprint (high to low in loading). Models considered include: hexagonal close packing of discs (hcp); Random Sequential Adsorption (RSA) of discs and rectangles; and oriented close packing. Equation 2 was used to calculate the footprint values.

Adsorption Model	θm	Adsorbed Dimensions	Footprint (m2/mg)	Loading (mg/m2)
end-on, close	1	4.5 × 14 nm	0.26	3.8
hcp, disc	0.91	10.5 nm disc	0.40	2.5
end-on, RSA	0.56	4.5 × 14 nm	0.47	2.1
side-on, close	1	10 × 14 nm	0.58	1.7
RSA, disc	0.547	10.5 nm disc	0.66	1.5
side-on, RSA	0.56	10 × 14 nm	1.04	0.96