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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Immunol. 2009 Aug 10;183(5):3400–3408. doi: 10.4049/jimmunol.0900838

Figure 2.

Figure 2

Neutrophil recruitment directly affected MMP-9 release into the airways. Balb/c mice were given a single injection of control Ab or RB65-8C5 (100 μg/mouse) 24h prior to a single intratracheal inhalation of PBS (40μl) or GC frass (40μg/40μl). 3 h later, mice were given a lethal dose of sodium pentobarbital and BAL fluid was harvested. Neutrophils were quantified following differential staining and a MMP-9 ELISA was performed on the BAL fluid. In all cases, means ± SEM (n=6 mice per group) were reported. Statistical significance was determined by ANOVA. A. Neutrophil influx into BAL fluid (*p<0.001) (nd=none detected). B. MMP-9 ELISA of BAL fluid (*p<0.001).