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MMP-9 levels were increased in BAL fluid of GC frass-treated mice. Balb/c mice were given a single intratracheal inhalation of PBS or GC frass and BAL fluid was harvested 18h later. MMP-9 activity was measured in the BAL fluid as determined by gelatin zymogram (A) or Western blot analysis using an antibody against the mature (active) form of MMP-9 (B).
