Table 1. Differential cell count in BAL fluid of sensitized and challenged wild type (C57Bl/6) and TLR2-deficient (TLR2-/-) mice.

C57Bl/6 mice were given intraperitoneal injections of PBS or GC frass with alum on day 0 and 7. Intratracheal inhalations of PBS or GC frass were performed on days 14 and 19. On day 22, BAL fluid was harvested and differential cell counts performed. These data represent 6-8 mice per group and are expressed as mean ± SEM of cell number × 10⁴. Cell counts were statistically significant between GC frass in the TLR2-deficient mice compared to wild type mice (*p<0.001, **p=0.003, as determined by ANOVA).

	Mac	Epi	Eos	Neut	Lymph
C57-PBS	3.4±1.1	3.7±1.4	0.03±0.02	0.03±0.02	0.02±0.006
TLR2-/- PBS	10.1±2.7	6.1±1.7	0.014±0.008	0.073±0.03	0.07±0.06
C57-frass	95.6±22.7	20.5±6.4	21.4±8.1	55.1±17.3	37.25±10.2
TLR2-/- frass	26.8±2.5*	3.3±0.3**	55.8±12.3**	3.8±1.0*	5.2±0.7*