Table 4. Differential cell count in BAL fluid of sensitized and challenged wild type (FVB) and MMP-9-deficient (MMP-/-) mice.

FVB or MMP-9-deficient mice were given intratracheal inhalations of PBS (40 μl) or GC frass (40 μg/40 μl) on days 0, 7, and 14. On day 17, BAL fluid was harvested and differential cell counts performed. These data represent 6-8 mice per group and are expressed as mean ± SEM of cell number × 10⁴. Cell counts were statistical significance between GC frass in the MMP-9-deficient mice compared to wild type mice (*p=0.002, **p<0.001, as determined by ANOVA).

	Mac	Epi	Eos	Neut	Lymph
FVB-PBS	1.1±0.3	1.7±0.4	0.01±0.004	0.01±0.005	0.009±0.005
MMP9-/- PBS	1.7±0.4	1.9±0.5	0.04±0.03	0.02±0.01	0.04±0.02
FVB-frass	13.7±2.4	8.2±2.2	53.9±17	0.5±0.1	3.3±0.5
MMP9-/- frass	15.3±1.7	8.3±2.7	139±17*	0.03±0.02**	3.5±1