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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Cancer Res. 2009 Jun 1;69(11):4870–4877. doi: 10.1158/0008-5472.CAN-08-4559

Figure 3.

Figure 3

Only cells that re-replicated their DNA experienced genotoxic stress and apoptosis. (A) Colon carcinoma HCT116 cells were transfected with either siGL2 or siGem. At 4 days post-transfection, cells not attached to the dish were isolated by centrifugation and subjected to the TUNEL assay. HCT116 cells treated with DNase I provided a TUNEL positive control. Normal skin D1 cells were treated likewise. (B) The indicated cells were treated with either siGL2 or siGem as in Fig. 1, and then subjected to Western immuno-blotting for the indicated protein (p53-P is p53 phosphorylated on serine 15). To demonstrate the effects of genotoxic stress, p53+/+ HCT116 cells were treated with 3 µM etoposide (ETP) for 16 hours in parallel with HCT116 cells treated with the same buffered solvent (DMSO). Longer exposures revealed p53 in HCT116 cells, and shorter exposures confirmed that p53 levels in AF11132 and MCF10A cells remained unchanged.