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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Cancer Res. 2009 Jun 1;69(11):4870–4877. doi: 10.1158/0008-5472.CAN-08-4559

Figure 4.

Figure 4

Depletion of geminin did not suppress proliferation of normal cells and did not induce apoptosis. (A) The total number of normal skin D1 cells transfected with either siGL2 (□) or siGem (■) at the indicated times post-transfection. (B) Cells treated as in (A) were subjected to Western immuno-blotting for geminin and actin. ‘Control’ indicates an unidentified protein that cross-reacted with anti-geminin antibody. (C) Cells in (A) were analyzed by FACS, as in Fig. 1. Percentage of cells with >4N DNA is shown. (D) The indicated cells were transfected with either siGem or siGL2. Six days later the total number of cells was counted, and the ratio of cells surviving siGem transfection relative to those surviving siGL2 transfection was determined.