Table 1.
Voltages of half inactivation (V0.5) and slopes (k) of fast and slow inactivation curves for BgNav1-1a BgNav1-1aF1817A, and BgNav1-1aY1824A sodium channels treated with indoxacarb or DCJW at depolarized holding potentials
| Control | Indoxacarb | DCJW | Metaflumizone | |||||
|---|---|---|---|---|---|---|---|---|
| V0.5 (mV) | k | V0.5 (mV) | k | V0.5 (mV) | k | V0.5 (mV) | k | |
| Fast Inact | ||||||||
| BgNav1-1a | -48.9 ± 0.8 | 5.9 ± 0.4 | -50.8 ± 1.5 | 6.4 ± 0.7 | -48.3 ± 0.9 | 5.2 ± 0.1 | -45.2 ± 1.3 | 4.0 ± 0.4 |
| BgNav1-1aF1817A | -48.4 ± 1.6 | 4.8 ± 0.2 | -52.2 ± 1.2 | 6.0 ± 0.3 | -49.9 ± 1.7 | 9.1 ± 2.0b | -46.3 ± 1.0 | 4.8 ± 0.2 |
| BgNav1-1aY1824A | -45.2 ± 1.6 | 5.5 ± 0.4 | -41.1 ± 1.2 | 4.9 ± 0.2 | -40.4 ± 1.0 | 5.0 ± 0.2 | -40.0 ± 0.9 | 5.0 ± 0.1 |
| Slow Inact | ||||||||
|---|---|---|---|---|---|---|---|---|
| BgNav1-1a | -54.6 ± 0.9 | 4.2 ± 0.2 | -64.6 ± 1.6 | 6.78 ± 0.4 | -71.8 ± 3.8b | 13.1 ± 1.0b | -51.1 ± 1.4 | 8.2 ± 0.2b |
| BgNav1-1aF1817A | -66.4 ± 0.4a | 3.8 ± 0.2 | -71.9 ± 0.9b | 5.2 ± 0.3 | -99.5 ± 1.0b | 10.1 ± 0.1b | -81.0 ± 1.6b | 13.5 ± 0.6b |
| BgNav1-1aY1824A | -61.4 ± 2.0a | 7.8 ± 0.8a | -57.0 ± 4.2 | 7.1 ± 1.4 | -57.8 ± 4.1 | 8.9 ± 2.1 | -54.8 ± 0.9 | 6.9 ± 0.6 |
a
significantly different from wild-type (BgNav1-1a, p < 0.05, n ≥ 3)
b
significantly different from control (p < 0.05, n ≥ 3)