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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):138–147. doi: 10.4049/jimmunol.182.1.138

FIGURE 3. Loss of λ5 protein in aged mice occurs at pro-B and pre-B cell stages.

FIGURE 3

(A) Cytoplasmic λ5 protein was stained and analyzed by flow cytometry in bone marrow c-kit+cμ- pro-B, c-kit+cμ+ early pre-B, and c-kit-cμ+ early pre-B cells in young and old BALB/c mice. Data shown are representative of 3 mice. Grey line=isotype control. Percent positive cells was determined with a cut-off as <2% positives in isotype controls. The change in mean fluorescent intensity (ΔMFI) was defined as (MFI experimental-MFI isotype control) and is shown in the upper left. (B) λ5 protein expression was determined in CD2-cμ- pro-B cells generated from young and old BALB/c mice upon bone marrow cell culture with IL-7 for 5-7 days. CD2- pro-B cells were separated by MACS sorting prior to lysate preparation. Proteins were assayed by Western blot at 2.5, 5, and 10 ×105 cell equivalents. Data are representative of 3 experiments.