Skip to main content
. 2009 May 27;29(21):7040–7052. doi: 10.1523/JNEUROSCI.0105-09.2009

Figure 5.

Figure 5.

The two-pore potassium leak channels TWIK1 and TASK1 are upregulated in G42 FS interneurons over postnatal development and exert increasing influence on membrane excitability. A, Average whole-cell currents evoked by a voltage ramp from −110 to −30 mV at P25 before and after bath application of 40 μm bupivacaine (left). Average bupivacaine-sensitive currents at P7, P10, and P25. Dashed lines indicate zero current and the potassium equilibrium potential (EK, right). B, Bupivacaine-sensitive conductances account for a greater proportion of the whole-cell resting conductance as G42 FS interneurons mature (left). Right panels represent the increasing developmental trajectory of kcnk3 transcript levels measured by microarray and TASK1 protein detected by immunostaining. C, Immunostaining for TASK1 at P10 (top row) and P25 (bottom row). G42 FS interneurons are labeled with GFP, and the amount and intensity of TASK1 signal coextensive with GFP signal both increase between P10 and P25. Arrows in the TASK panels indicate the location of G42 somata in the same FOV. Scale bar, 40 μm.