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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: J Immunol. 2006 May 15;176(10):5720–5724. doi: 10.4049/jimmunol.176.10.5720

FIGURE 5.

FIGURE 5

Activation and requirement of ASK1 in LPS signaling: A, U373 cells were stimulated with 1 µg/ml LPS or TNF-α for the indicated times, and blots were probed with anti-phospho-T845 ASK1 or ASK1 Abs. CTL, control. B, Peritoneal macrophages from WT and ASK1−/− mice were stimulated with 1 µg/ml LPS for the indicated times, and ISG54 and GAPDH mRNA levels were analyzed by RT-PCR. C, WT and ASK1−/− MEFs were transfected with murine TRIF (mTRIF) and/or IRF3 and ISRE luciferase. After 24 h, luciferase activity was analyzed. A representative of three independent experiments is shown. b-gal, β-galactosidase.