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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: J Biol Chem. 2000 Nov 20;276(3):2083–2087. doi: 10.1074/jbc.C000739200

Fig. 2. The cvt17Δ strain is defective in autophagy.

Fig. 2

A, log phase cultures grown in 1% yeast extract, 2% peptone, 2% glucose (YPD) were shifted to medium lacking nitrogen (SD-N). Viability was determined by plating aliquots on YPD plates. B, indicated strains were shifted to SD-N as in A, with aliquots taken at indicated times. Crude lysates were subjected to immunoblot analysis with anti-API antiserum.