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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Nature. 2008 Jul 23;454(7207):1005–1008. doi: 10.1038/nature07170

Figure 1. EspFU is a potent activator of N-WASP.

Figure 1

a, EspFU is secreted from EHEC into a host cell where it stimulates actin polymerization through endogenous N-WASP and Arp2/3, leading to pedestal formation. b, N-WASP is basally autoinhibited, but can be activated by inputs like Cdc42. c, Multiple endogenous activators are necessary to potently activate N-WASP. EspFU accomplishes this with a single input. d, EspFU potently activates N-WASP in an in vitro pyrene-actin polymerization assay. Maximal rate (50 nM unregulated N-WASP WCA) shown in gray dotted line. All other reactions contain 50 nM mini N-WASP (B-GBD-linker-WCA). e, Sequence of EspFU. f, Mapping minimal binding fragments of EspFU and N-WASP with a glutathione S-transferase (GST) pulldown assay.