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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Nature. 2008 Jul 23;454(7207):1005–1008. doi: 10.1038/nature07170

Figure 2. EspFU mimics the N-WASP autoinhibitory C helix.

Figure 2

a, Structure of autoinhibited fragment of WASP7, highlighting the C helix (green) and three critical side chains (red). b, EspFU can competitively disrupt the N-WASP autoinhibitory interaction (in trans) in a GST-pulldown assay. c, Alanine scanning mutagenesis shows similar pattern of critical residues in N-WASP C helix and EspFU (repeat 4, residues 1–17) (assayed by binding to GBD in a GST-pulldown assay). d, Model comparing N-WASP activation by Cdc42 and EspFU. e, PAK kinase assay showing phosphorylation of myelin basic protein (MBP). PAK2 alone is autoinhibited – Cdc42 activates it, but EspFU does not.