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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Nature. 2008 Jul 23;454(7207):1005–1008. doi: 10.1038/nature07170

Figure 3. Multiple repeats of EspFU are required for potent activation of actin polymerization.

Figure 3

a, Activities of EspFU fragments in a pyrene-actin polymerization assay. Data are fit to single exponential curves using MATLAB (MathWorks), with a small linear term subtracted to account for EspFU sequestration of actin and/or Arp2/3. b, Visualization of fluorescently-labeled actin around beads coated with EspFU fragments. Images were acquired four minutes after initiation. c, NIH 3T3 cells expressing GFP-actin and EspFU fused to a transmembrane protein (CD16/7), which is clustered with a rhodamine-tagged antibody26. Inset in each image is a portion of that cell (white box) shown at higher magnification. d, Results from quantification of clustered cells, shown in two metrics. Bars represent mean ± s.e.m. of 10 cell images. e, Activity correlates with ability of EspFU fragments to bind at least two N-WASP proteins simultaneously.