Figure 7.

Structural microenvironments of residues exhibiting extreme positions along PC2. (A) Arg⁴⁷¹ (PDB id 1ifrA), near a maximum value of PC2, and its immediate unfolding neighbors are shown as pink sticks. A large amount of polar surface area (colored red) surrounds these residues. (B) Leu¹⁸⁰ (PDB id 1gsmA), near a minimum in PC2, and its unfolding neighbors are shown as pink sticks. Note the surrounding surface area is almost completely apolar (blue). In both panels, gray area corresponds to surface that is neither highly apolar nor highly polar. Polar area is defined as residue types R, K, H, E, D, N, Q, T, S, and C, and apolar area is defined as residue types A, G, V, I, L, F, and M.