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. 2009 Sep 2;97(5):1390–1397. doi: 10.1016/j.bpj.2009.06.029

Table 3.

Excited state lifetime distribution of UDP-MurNAc-pentapeptide-DNS (40 μM), DNS-lipid I, and DNS-lipid II (5 μM each)

Sample α1 α2 α3 τ1 (ns) τ2 (ns) τ3 (ns) 〈τ〉 (ns)
UDP-MurNAc-pentapeptide-DNS 0.22 ± 0.02 0.03 ± 0.02 0.75 ± 0.02 0.20 ± 0.06 1.38 ± 0.53 3.58 ± 0.12 2.77 ± 0.06
DNS-lipid I 0.19 ± 0.01 0.06 ± 0.01 0.75 ± 0.02 0.92 ± 0.14 4.23 ± 0.31 14.28± 0.09 11.16 ± 0.15
DNS-lipid I/clausin 0.21 ± 0.02 0.14 ± 0.01 0.65 ± 0.02 0.65 ± 0.10 2.45 ± 0.47 11.88 ± 0.24 8.20 ± 0.05
DNS-lipid II 0.20 ± 0.01 0.08 ± 0.01 0.72 ± 0.01 0.66 ± 0.15 3.33 ± 0.38 14.32 ± 0.03 10.69 ± 0.28
DNS-lipid II/clausin 0.23 ± 0.01 0.12 ± 0.03 0.65 ± 0.03 0.81 ± 0.11 3.19 ± 1.05 11.98 ± 0.25 8.36 ± 0.25

Microcuvettes of 140 μL were used. Clausin concentration, 130 μM; DPC, 1%; excitation, 375 nm; emission, 525 nm + Schott KV418 cutoff filter.

Amplitude-averaged lifetime was defined as τ=iαiτi.