Table 4.

Fluorescence anisotropy decay parameters of UDP-MurNAc-pentapeptide-DNS (40 μM), DNS-lipid I, and DNS-lipid II (5 μM each)

Sample	β1	β2	θ1 (ns)	θ2 (ns)	At=0	ωmax (°)
UDP-MurNAc-pentapeptide-DNS	-	0.343 ± 0.031	-	0.28 ± 0.02	0.343 ± 0.031	-
DNS-lipid I	0.092 ± 0.021	0.198 ± 0.004	0.7 ± 0.1	3.6 ± 0.1	0.289 ± 0.017	23 ± 3
DNS-lipid I/clausin	0.084 ± 0.008	0.188 ± 0.009	1.8 ± 0.5	9.4 ± 0.3	0.272 ± 0.009	26 ± 2
DNS-lipid II	0.105 ± 0.020	0.182 ± 0.008	0.8 ± 0.2	4.5 ± 0.2	0.287 ± 0.048	23 ± 3
DNS-lipid II/clausin	0.079 ± 0.005	0.174 ± 0.002	1.7 ± 0.1	8.9 ± 0.1	0.253 ± 0.003	28 ± 2

Microcuvettes of 140 μL were used. Clausin concentration, 130 μM; DPC, 1%; excitation, 375 nm; emission, 525 nm + Schott KV418 cutoff filter. The semi-angle of the wobbling-in-cone motion ω_max was calculated as: $\frac{A_{t=0}}{A_0}={\left[\raisebox{1ex}{$1$}\!\left/ \!\raisebox{-1ex}{$2$}\right.\text{cos}{\omega }_{\text{max}}\left(1+\text{cos}{\omega }_{\text{max}}\right)\right]}^2$(60) with the intrinsic anisotropy A₀ = 0.370 (39,42).