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. 2009 Sep 2;97(5):1381–1389. doi: 10.1016/j.bpj.2009.06.040

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Effect of temperature on phase segregation in native pulmonary surfactant and membranes reconstituted from its fractions Segregation of phases at temperatures below (25°C) and above (40°C) of the thermotropic transition of surfactant membranes was observed by confocal microscopy of GUVs produced from native pulmonary surfactant (NPS) or its fractions (NPSE, NPSL, NPSP), doped with 0.1 mol % each of the probes DiIC18 (red) and Bodipy-PC (yellow-green). The scale bar represents 10 μm for all images except that of the GUV from NPS and observed at 40°C, which has its own smaller scale bar (also representing 10 μm).