Table 1.
Oligonucleotides used in this study
| Name | Sequence | Application |
| cbh1SF | 5'-TCGGCCTGCACTCTCCAATCC-3' | PCR fragments used as probes for northern blots |
| cbh1SR | 5'-TGGAGTCCAGCCACAGCATG-3' | |
| 18SRF | 5'-GGTGGAGTGATTTGTCTG-3' | |
| 18SRR | 5'-CTTACTAGGGATTCCTCG-3' | |
| hfb1F | 5'-CTGAACACCTCCAGTCAAC-3' | |
| hfb1R | 5'-TATTGGAGAATAAGAATGGC-3' | |
| hfb2F | 5'-TCAAGATGCAGTTCTTCG-3' | |
| hfb2R | 5'-CATTGCTTTAGAAGGTGC-3' | |
| gna1aa5F | 5'-ACACGAACGCCCAAATCTG-3' | Construction of vector pBgna1Q204L |
| gna1aa5R | 5'-CTTTCGCTCAGATCGCTG-3' | |
| gna1aa3F | 5'-GCCAGCGATCTGAGCGAAAGAAG-3' | |
| gna1aa3R | 5'-GGGCGACGGCACATTAACATAG-3' | |
| gna1aa5NF | 5'-atgtcgacCAAATCTGCGTCTTCCACAC-3' | |
| gna1aa3NR | 5'-atgggcccCGGCACATTAACATAGCTTG-3' | |
| gna1F | 5'-TAAGCGAAGGTTCTCCTG-3' | cDNA amplification |
| gna1R | 5'-ATTGGCATTTCATTCCTC-3' | |
| gna1D5F | 5'-atgggcccCATCTCCACAGTTTCCAGG-3' | Construction of vector pDELgna1 |
| gna1D5R | 5'-atgtcgacTGTTGGCGACTGTGAATG-3' | |
| gna1D3F | 5'-atggatccTTAGACGACTTGTTGATTGTTG-3' | |
| gna1D3R | 5'-attctagaCCCAATGCTTATAGAACATC-3' | |
Mutated bases are given in bold, artificially introduced restriction sites are given in lower case letters.
PCR = polymerase chain reaction.