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. 2009 Sep 10;9:230. doi: 10.1186/1471-2148-9-230

Figure 2.

Figure 2

Phylogenetic relationships of N. vectensis NRs (NvNRs) depicted by a maximum-likelihood tree with percent bootstrap support of 1000 bootstraps for nodes of evolutionary significance. The alignment was constructed using the DBD and a portion of the LBD (additional details in text). Monophyletic relationships for bilaterian sequences in particular families or subfamilies were recovered in all cases but one (NR2E) and are depicted as horizontal triangle. A full tree with all taxa depicted as individual branches is presented in Additional File 2. The tree was rooted with NR family 2 because this family is monophyletic, represents a defined nuclear receptor family, and includes what is likely the original nuclear receptor, represented by an HNF4-like homolog from the sponge Amphimedon queenslandica [1]. Most N. vectensis NRs group within NR family 2 and many (e.g., NvNR11- NvNR14) are supported as independent radiations of subfamilies within this family. N. vectensis also has a NR related to GCNF (NR family 6), but with low support. Three NRs did not group with any previously described family (NvNR1-3). These genes may represent ancestral genes that later diversified into one or more of the NR 1, 3, and 4 families.