Figure 3.

Developmental expression of Nematostella vectensis nuclear receptors (NR1-NR17) and putative housekeeping genes (18S and GAPDH). Expression measured using qPCR, as described in text. Expression in molecules per μl cDNA indicated on the y-axis. Bar indicate the mean ± standard error of 3-5 biological replicates. The first bars ("cleavage") represents a single sample, which was not included in statistical analysis. Expression patterns were analyzed with a one-way ANOVA followed by pairwise comparisons with Tukey's Honestly Significant Difference Test. Letters indicate groups that were different at a level of p = 0.05. GAPDH showed variable expression over the sampled stages and was not used as a standard for comparing gene expression of NRs among developmental stages.