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. 2009 Sep 16;10:292. doi: 10.1186/1471-2105-10-292

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Copyright © 2009 Rubin; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Plots of within-chip log amplitude z-scores for two more HG-U133ASpike-in Experiment "Unanticipated Positives". These are two more genes which are not in the Affymetrix original list of spiked-in genes, and which were considered for inclusion by McGee and Chen but ultimately rejected. 203173_s_at (shown on the left) is similar in profile to 204890_s_at and 204991_s_at. The profiles are somewhat different for 203060_s_at (on the right), but still do not appear to be compatible with the hypothesis that they come from probe sets with identical probe-level distributions for experimental conditions 1 (cyan) and 14 (red). These genes are also obvious unanticipated positive candidates.