HSQC analysis of crude heparin (black) compared with purified heparin (gold). Whereas crude heparin contains a number of components, purified heparin contains largely (or only) heparin. Signals associated with CS A and C are identified in red; those arising from HA are shown in light blue. Signals from DS are shown in green, whereas those that arise primarily or exclusively from heparin are identified in black. ANS, N-sulfoglucosamine; ANAc, N-acetylglucosamine; I, iduronic acid; G, glucuronic acid; LR, linkage region.