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Surface markers and viability stain. All antibodies and 7-AAD used were purchase from BD Pharmingen.
| Marker | Main marked cells |
|---|---|
| CD3 | T lymphocytes |
| CD4 | Th lymphocytes |
| CD8 | Tc lymphocytes |
| CD19 | B lymphocytes |
| PanNK (DX5) | NK lymphocytes |
| CD25 | Activated lymphocytes |
| 7-AAD | Non-viable cells |
