Table 3.

CD25 expression in macrophage-stimulated lymphocytes in contact with B16F10 cells.

Mϕ/Ly
7-AAD viability
	Mean	Transformed mean	P/F
Control	193	13.927	0.28
Treated	179	12.898
CD25 expression
	Mean	Transformed mean	P/F
Control	1260.33	35.23	0.72
Treated	1150	33.788
Mϕ/Ly*
7-AAD viability
	Mean	Transformed mean	P/F **
Control	1074.66	32.708	0.009
Treated	395.67	19.647
CD25 expression
	Mean	Transformed mean	P/F **
Control	238.666	14.832	0.007
Treated	1119	33.323
B16F10/Ly-Mϕ
7-AAD viability
	Mean	Transformed mean	P/F
Control	2368.7	48.3168	0.899
Treated	2985.583	54.0464
CD25 expression
	Mean	Transformed mean	P/F *
Control	2295.8	59.6266	0.0113
Treated	3710.416	47.2793

Percentage data, obtained from light microscopy analysis, was transformed as described in Materials and Methods. Statistical significance of transformed data was determined by a single-factor ANOVA. Data are representative of three independent co-culture experiments.