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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Dev Cell. 2009 Aug;17(2):234–243. doi: 10.1016/j.devcel.2009.07.008

Figure 3. ESCRT-II is not required for Vps20 membrane localization in yeast.

Figure 3

(A-B) The designated VPS20-GFP alleles were transformed into the indicated yeast strain and their localization imaged by fluorescence microscopy as described in the experimental procedures. (C) Binding of VPS20 and human ESCRT-II to PC:PE:Cholesterol:PI(3)P liposomes monitored by FRET. Error ranges reported in the Kd values were derived from the errors in the non-linear best fit. Error bars in the data points shown in (C) were derived from the propagation of the estimated error in the measurement of ΔImax. All experiments were carried out twice.